abstract：:Human hair analysis is now recognized for evaluating someone exposure to xenobiotics: drugs of abuse, pharmaceuticals and polluants. This paper describes analytical methods than can be used by biologists. For drugs of abuse after decontamination, hydrolysis of hair, selective extraction and derivatization, determinati...

journal_title：Annales de biologie clinique

authors： Goullé JP,Kintz P

更新日期：1997-09-01 00:00:00

abstract：:Rheumatoid arthritis is the most common and most severe form of inflammatory arthritis. Its exact aetiology remains unknown but the combination of various risk factors is involved. Two non mutually exclusive concepts have been suggested: one based on the primary contribution of T cells and the other on that of mesench...

journal_title：Annales de biologie clinique

authors： Miossec P

更新日期：1997-07-01 00:00:00

abstract：:Haemochromatosis is the most common genetic disease in individuals of Northern European origin. This disorder of iron metabolism, for which the molecular basis remains poorly understood, is characterized by an excessive absorption of dietary iron through the duodenal mucosa. Progressive iron loading of parenchymal org...

journal_title：Annales de biologie clinique

authors： Jouanolle AM,David V,Le Gall JY

更新日期：1997-05-01 00:00:00

abstract：:The introduction of the CRM 470 in 1993 (certified reference material for 14 serum proteins) and its utilization by industrial companies for cross-calibrating their commercial standards has been an important break-through in protein standardization. This improvement has been clearly illustrated by the last national qu...

journal_title：Annales de biologie clinique

authors： Bienvenu J,Doche C,Later R,Manceaux JC,Pontet F,Pressac M,Vassault A,Dumont G

更新日期：1997-01-01 00:00:00

abstract：:Cystic fibrosis (CF) is the most common severe recessive disease in Caucasians. The gene responsible for the disease encodes a protein named cystic fibrosis transmembrane conductance regulator, which is predicted to function as a cAMP-regulated chloride channel. Reduced chloride secretion is at the basis of insufficie...

journal_title：Annales de biologie clinique

authors： Ferrari M,Cremonesi L

更新日期：1996-01-01 00:00:00

abstract：:Therapeutic monitoring of patients participating in methadone maintenance programmes requires the qualitative or quantitative determination of methadone in urine before and during treatment. Generally immunoassay techniques are used for this determination, but more and more often measurement of plasma or serum levels ...

journal_title：Annales de biologie clinique

authors： Dessalles MC,Gasdeblay S,Mahuzier G

更新日期：1996-01-01 00:00:00

abstract：:The aim of this study was to evaluate a new automated fluorimetric enzyme immunoassay for CK-MB using the Vidas bioMérieux. The results for sera from 222 patients were compared with those obtained using two other methods of CK-MB determination in mass units (Stratus II Dade, Magic Lite Analyzer II Ciba Corning). Satis...

journal_title：Annales de biologie clinique

authors： Ayed SB,Boukezia N,Foglietti MJ,Bernard M

更新日期：1996-01-01 00:00:00

abstract：:Multidrug resistance (MDR) phenotype expression was evaluated retrospectively in 87 patients with acute myeloid leukemia (AML), 69 with de novo AML, ten with relapsed AML and eight with AML secondary to myelodysplastic syndrome (MDS). MDR phenotype, characterized by P-glycoprotein expression (MRK16 monoclonal antibody...

journal_title：Annales de biologie clinique

authors： Guerci A,Merlin JL,Missoum N,Feldmann L,Marchal S,Guerci O

更新日期：1996-01-01 00:00:00

abstract：:This paper describes the assessment of the homogeneity and stability of a purified and lyophilized human thyroglobulin (Tg), and characterizes its immunoreactivity. The purified and lyophilized Tg is intended to be used as a primary reference material to establish calibration of working serum based reference material....

journal_title：Annales de biologie clinique

authors： Feldt-Rasmussen U,Profilis C,Colinet E,Black E,Bornet H,Bourdoux P,Carayon P,Ericsson UB,Koutras DA,Lamas de Leon L,DeNayer P,Pacini F,Palumbo G,Santos A,Schlumberger M,Seidel C,Van Herle AJ,De Vijlder JJ

更新日期：1996-01-01 00:00:00

abstract：:The impact of the major interferents (hemolysis, bilirubin, turbidity), on the quality of biochemical tests, was evaluated on multiparametric analysers (CL 7200 Shimadzu, Japan/Ciba-Corning, France; AU 5231 and AU 5223 Olympus, Japan/bioMérieux, France), according to the SFBC instructions. Interferences were detected ...

journal_title：Annales de biologie clinique

authors： Frémont S,Combe AM,Mecrin M,Tronel H,Galland AV,Scholl C,Nicolas JP

更新日期：1996-01-01 00:00:00

abstract：:Salivary cortisol was determined with four commercially available immunoassays: one enzyme-immunoassay (Cortisol Biotrol), two fluoro-immunoassays (TDX, Abbott; Stratus, Baxter) and one radioimmunoassay (Coat-A-Count, Behring). In order to improve the sensitivity of these immunoassays, it was necessary to increase sam...

journal_title：Annales de biologie clinique

authors： Nahoul K,Patricot MC,Bressot N,Penes MC,Revol A

更新日期：1996-01-01 00:00:00

abstract：:Since 1989 many kits have been commercialized for measuring estradiol by non-isotopic immunoassays which largely contributed to the diffusion of this parameter. Rapidly, in quality control schemes, a wide dispersion of results has been observed. In this paper, the kits' characteristics (precision, accuracy and detecti...

journal_title：Annales de biologie clinique

authors： Patricot MC,Badonnel Y,Bugugnani MJ,Collignon I,Delvigne L,Lacroix I,Mathian B

更新日期：1995-01-01 00:00:00

abstract：:A recently commercialized dot-blot (Cyto-Dot, BMD) offered a new method for the detection of three autoantibodies (Ab) anti-Jo-1, anti-M2, and anti-ribosomal protein although their only common point is the cytoplasmic localisation of their respective antigen. These Ab are detected by indirect immunofluorescence (IF) (...

journal_title：Annales de biologie clinique

authors： Roussel B,Dubel L,Kantara-Doukoure M,Bernard M,Johanet C

更新日期：1995-01-01 00:00:00

abstract：:We have compared serum galactosyltransferase activity in pregnant women and in newborn children. Subjects in this study were 75 women at different stages of pregnancy and 60 newborns, including premature infants. Activity ratios in pregnant women are based on the period of gestation, expressed in weeks of amenorrhea. ...

journal_title：Annales de biologie clinique

authors： Bar E,Sichel F,Guerin F,Gauduchon P,Asselin D,Le Talaer JY

更新日期：1995-01-01 00:00:00

abstract：:Horseradish peroxidase is often used as an antibody-coupled enzyme and several procedures have been developed to obtain IgG-peroxidase conjugates. The most widely used are coupling with periodate or glutaraldehyde. To compare the efficiency of these methods, the authors conducted periodate coupling or glutaraldehyde c...

journal_title：Annales de biologie clinique

authors： Tresca JP,Ricoux R,Pontet M,Engler R

更新日期：1995-01-01 00:00:00

abstract：:Leukotrienes constitute a class of potent biological mediators of inflammation and anaphylaxis. However, their routine assay in various biological fluids is restricted by the complexity of the methodology. Previously this could only be performed by research laboratories with high pressure liquid chromatography and rad...

journal_title：Annales de biologie clinique

authors： Chabane MH,Le Vraux V,Le Provost AM,Leynadier F

更新日期：1995-01-01 00:00:00

abstract：:A previous multicentric study set up by the Société française de biologie clinique has emphasized the usefulness of a standardized procedure for the determination by high performance liquid chromatography of alpha-tocopherol in serum or plasma. In our study, we have tested every step of the different published procedu...

journal_title：Annales de biologie clinique

authors： Jezequel-Cuer M,Le Moël G,Mounié J,Peynet J,Le Bizec C,Vernet MH,Artur Y,Laschi-Loquerie A,Troupel S

更新日期：1995-01-01 00:00:00

abstract：:A multiresistant Salmonella typhi (S typhi, strain 302) was isolated from a blood culture of a patient in the Infectious Diseases department of Rabta Hospital in Tunis. The following tests were carried out: antibiotic susceptibility testing by the agar diffusion method; determination of the minimum inhibitory concentr...

journal_title：Annales de biologie clinique

authors： Ben Hassen A,Meddeb M,Ben Chaabane T,Zribi M,Ben Redjeb S

更新日期：1994-01-01 00:00:00

abstract：:Diosmin (DI) 3'5,7-trihydroxy-4'-methoxy flavone rutinoside is a member of the flavonoid family, some of whom have antioxidant or free radical scavenger properties. Paw oedema induced by doxorubicin in rats is reduced by DI as observed by SAUVAIRE et al (1989). This suggests a free radical scavenger activity for DI. I...

journal_title：Annales de biologie clinique

authors： Dumon MF,Freneix-Clerc M,Carbonneau MA,Thomas MJ,Perromat A,Clerc M

更新日期：1994-01-01 00:00:00

abstract：:Lysosomal storage diseases are genetically determined metabolic diseases characterized by dysmorphology and dysfunction of the lysosomal system. The lysosomal pathology can have different causes; these are (i) the deficiency of a lysosomal enzyme or subunit thereof, (ii) the deficiency of a protein assisting one or mo...

journal_title：Annales de biologie clinique

authors： Reuser AJ,Kroos MA,Visser WJ,Willemsen R

更新日期：1994-01-01 00:00:00

abstract：:Serum carbohydrate-deficient-transferrin (CDT) was measured by a micro anion-exchange chromatography/enzyme immunoassay. Results obtained on 245 sera analyzed in four laboratories were compared. Moreover, one laboratory used a commercial kit with ready-to-use microcolumns and a radioimmunoassay for measuring eluted CD...

journal_title：Annales de biologie clinique

authors： Vernet M,Renversez JC,Revenant MC,Sotta C,charlier De Bressing C,Benoit MO,Guillemin C,Paris M,Plomteux G

更新日期：1994-01-01 00:00:00

abstract：:Glycated hemoglobin can be assayed by methods using different principles, whose performances vary and are difficult to control. The most widely used techniques measure either specifically glycated A1c hemoglobin (HbA1c) or total glycated hemoglobin. They are reviewed in detail in this paper and standardization problem...

journal_title：Annales de biologie clinique

authors： Gillery P,Guillemin C,Delpech M

更新日期：1994-01-01 00:00:00

abstract：:Laboratory diagnosis of pheochromocytoma must give evidence of increased catecholamine production. This requires measurement of catecholamines and their metabolites (normetanephrine NMN, metanephrine MN and/or VMA) in urine or in plasma. The various assays can be also performed during dynamic test that stimulate or in...

journal_title：Annales de biologie clinique

authors： Peyrin L,Mornex R

更新日期：1993-01-01 00:00:00

abstract：:In order to increase the versatility of phage display technology, it is desirable to be able to impose some structural constraints on the peptides that are presented by the phage particles. This is currently not feasible since the conformation of the capsid proteins, used to link the foreign peptide to the phage, are ...

journal_title：Annales de biologie clinique

authors： Santiago Vispo N,Felici F,Castagnoli L,Cesareni G

更新日期：1993-01-01 00:00:00

abstract：:Red cell ferritin is a residue of erythroblast ferritin. It reflects the balance between the iron supply to the erythroid marrow and the need for haemoglobin synthesis. Erythrocyte ferritin can be measured in haemolysates after discarding plasma and leucocytes by different methods. The decrease in erythrocyte ferritin...

journal_title：Annales de biologie clinique

authors： Guillemin C,Revenant MC,Vernet M,Dezier JF,Charlier C,Paris M,Pressac M

更新日期：1993-01-01 00:00:00

abstract：:Currently, the dose of anticancer drugs is adjusted according to patient body surface area, although the best criterion for dose adjustment seems to be the plasma concentration of the drug, since a correlation has been established between plasma concentration and efficacy for several drugs. We report here similar resu...

journal_title：Annales de biologie clinique

authors： Desoize B,Maréchal F,Cattan A

更新日期：1993-01-01 00:00:00

abstract：:Monoclonal antibodies have been raised against porcine LH receptor and allowed to clone the corresponding messenger RNA from testicular cells. Cross hybridisation with the LH receptor clone allowed to isolate a clone corresponding to the human TSH receptor from thyroids. The structure of both receptors have been deter...

journal_title：Annales de biologie clinique

authors： Misrahi M,Loosfelt H,Atger M,Vu Hai MT,Gross B,Meduri G,Jolivet A,Milgrom E

更新日期：1992-01-01 00:00:00

abstract：:Second generation hepatitis C virus (HCV) Elisas are currently in use in Europe and have been submitted for approval in the United States. These new assays contain additional antigens from the putative nucleocapsid and NS-3 regions of the HCV genome in addition to the c100-3 antigen present in first generation Elisas....

journal_title：Annales de biologie clinique

authors： Polito AJ,DiNello RK,Quan S,Andrews W,Rose J,Lee F,Nelles M,Lee S

更新日期：1992-01-01 00:00:00

abstract：:Spontaneous red blood cell (RBC) fragmentation occurs in some membrane erythropathies like hereditary elliptocytosis (HE); this phenomenon is produced in normal RBC by heating at 49 degrees C, but not at temperatures below this limit; fragmentation is usually quantified by counting the number of fragments/1000 RBC und...

journal_title：Annales de biologie clinique

authors： Guetarni D,Zerhouni F,Beldjord K,Henni T,Godet J,Colonna P

更新日期：1992-01-01 00:00:00

abstract：:Determination of plasma delta-aminolaevulinic acid levels is of interest for the diagnosis of disorders of heme biosynthesis. We describe a reliable method for the determination of this parameter, based on a modification of the original method of Mauzerall and Granick. Analytical criteria are defined and the reference...

journal_title：Annales de biologie clinique

authors： Goujon R,Philibert E,Bernard P,Normand J,Boucherat M

更新日期：1992-01-01 00:00:00

abstract：:High resolution proton nuclear magnetic resonance (NMR) spectroscopy is a new analytical technique which allows to readily identify and quantitate a variety of key metabolites in cerebrospinal fluid (CSF) in relation to normal and pathological brain activity. Proton NMR spectroscopy can be performed on native CSF, wit...

journal_title：Annales de biologie clinique

authors： Kriat M,Nicoli F,Vion-Dury J,Confort-Gouny S,Dano P,Grisoli F,Gastaut JL,Cozzone PJ

更新日期：1991-01-01 00:00:00

abstract：:Immunoassay quantitation of human antibody depends on the availability of reference sera that have been characterized in terms of their antibody content. This report expands upon the concept of "cross-calibration", using reference (dose-response) curves that have been generated with heterologous antibody as a means of...

journal_title：Annales de biologie clinique

authors： Hamilton RG

更新日期：1991-01-01 00:00:00

abstract：:Mycoplasma hominis, a mycoplasma potentially isolated from the genital tract of healthy women, seems to have some role in pelvic inflammatory diseases (PID). Three serologic techniques were developed to detect antibodies to M. hominis: ELISA (IgG, IgM), microimmunofluorescence (MIF) and Western blot. Rabbit and human ...

journal_title：Annales de biologie clinique

authors： Constans J,Poutiers F,Renaudin H,Leng JJ,Bebear C

更新日期：1991-01-01 00:00:00

abstract：:A new automated system for identification of anaerobes in 4 hours, ATB 32 A, was evaluated with 124 strains isolated in routine and 10 collection strains, and was compared with techniques commonly used in the hospital. The system allowed identification of 89.5% (111/124) of clinical strains, including 6.4% (7/124) str...

journal_title：Annales de biologie clinique

authors： Roger F,Roger A,Caniaux I

更新日期：1991-01-01 00:00:00

abstract：:48 Tunisian people suffering from the IDDM auto-immune disease were compared to 35 control healthy persons for the polymorphisms of the complement BF, C2 and C4 proteins and genes, of the IgG (Gm allotypes) as well as of the TNF alpha and TCR C beta genes. Our study shows that the BFF1-C4A3-C4BQO and BFS-C4AQ0-C4B1 co...

journal_title：Annales de biologie clinique

authors： Ben Salem M,Ghanem N,Kneissi T,Abbal M,Dugoujon JM,Ben Chibani J,Ellouze F,Lefranc G

更新日期：1991-01-01 00:00:00

abstract：:In this study the authors compare two automated coagulation instruments: the koagulab-40A (Orthodiagnostics Systems) and l'ACL-100 (Instrumentation Laboratory) using two different methods of detection, photo-optical and nephelemetric respectively. The three parameters studied are: prothrombin time (PT) (82 samples), a...

journal_title：Annales de biologie clinique

authors： Barbe D,Bauduer F,Tanguy A

更新日期：1991-01-01 00:00:00

abstract：:Heterogeneous immunoassays are very sensitive and only limited in terms of performance by non specific binding. They require separation of free from bound fractions and concomitant use of a solid phase coated with an immunoreactive component (i.e. immunosorbent). The improvement of these key immunosorbents is crucial ...

journal_title：Annales de biologie clinique

authors： Delagneau JF,Masseyeff R

更新日期：1990-01-01 00:00:00

abstract：:The aging process is an universal phenomenon. All individuals of a given species are dead by some age characteristic of the species. In spite of many biological and clinical observations on the changes that occur during aging, understanding about the causes of aging or the mechanisms determining "the life span potenti...

journal_title：Annales de biologie clinique

authors： Crastes de Paulet A

更新日期：1990-01-01 00:00:00

abstract：:Determination of sex-hormone-binding-globulin was performed by three methods: binding, electroimmunodiffusion and DELFIA methods. Results of normal and pathological values correlated well with the three techniques. ...

journal_title：Annales de biologie clinique

authors： Patricot MC,Mathian B,Later F,Revol A

更新日期：1990-01-01 00:00:00

abstract：:Anti native DNA antibodies (anti nDNA Ab), which are a highly specific feature of systemic lupus erythematosus (SLE) were measured by 3 methods: an enzyme linked immunosorbent assay (ELISA), an indirect immunofluorescence test on Crithidia luciliae (IFCL) and the Farr assay (reference test). 114 sera from patients wit...

journal_title：Annales de biologie clinique

authors： Laplanche S,Diemert MC,Musset L,Abuaf N,Galli J

更新日期：1990-01-01 00:00:00